Image and Video Processing (eess.IV)

Abstract: Organoids are self-organized 3D cell clusters that closely mimic the architecture and function of in vivo tissues and organs. Quantification of organoid morphology helps in studying organ development, drug discovery, and toxicity assessment. Recent microscopy techniques provide a potent tool to acquire organoid morphology features, but manual image analysis remains a labor and time-intensive process. Thus, this paper proposes a comprehensive pipeline for microscopy analysis that leverages the SegmentAnything to precisely demarcate individual organoids. Additionally, we introduce a set of morphological properties, including perimeter, area, radius, non-smoothness, and non-circularity, allowing researchers to analyze the organoid structures quantitatively and automatically. To validate the effectiveness of our approach, we conducted tests on bright-field images of human induced pluripotent stem cells (iPSCs) derived neural-epithelial (NE) organoids. The results obtained from our automatic pipeline closely align with manual organoid detection and measurement, showcasing the capability of our proposed method in accelerating organoids morphology analysis.

Abstract: Chest X-rays (CXRs) are a medical imaging modality that is used to infer a large number of abnormalities. While it is hard to define an exhaustive list of these abnormalities, which may co-occur on a chest X-ray, few of them are quite commonly observed and are abundantly represented in CXR datasets used to train deep learning models for automated inference. However, it is challenging for current models to learn independent discriminatory features for labels that are rare but may be of high significance. Prior works focus on the combination of multi-label and long tail problems by introducing novel loss functions or some mechanism of re-sampling or re-weighting the data. Instead, we propose that it is possible to achieve significant performance gains merely by choosing an initialization for a model that is closer to the domain of the target dataset. This method can complement the techniques proposed in existing literature, and can easily be scaled to new labels. Finally, we also examine the veracity of synthetically generated data to augment the tail labels and analyse its contribution to improving model performance.