Rymuza, J.; Zhang, Q.; Bujko, M.

Neuroendocrine pituitary tumors (PitNETs) are classified based on clinical manifestation and expression of pituitary cell lineage-specific transcription factors (TFs) and hormones. A subtype of acromegaly-related tumors was found to express PIT-1 and SF-1 TFs, two markers of distinct pituitary cells. They were considered as multilineage or \'somatogonadotoph\' tumors. The aim of our study was to determine their identity and cell type origin by extensive transcriptomic analysis. For this purpose, we analyzed the RNA sequencing (RNAseq) data from 546 PitNETs (including 193 acromegaly-related tumors) and single cell RNAseq data from somatotroph and gonadotroph tumors and normal pituitary. Somatrotroph PitNETs co-expressing PIT-1 and SF-1 TFs were found in each included RNAseq dataset. Their transcriptomic profile and activity of pituitary TFs closely resembles other somatotroph tumors, but they substantially differ from gonadotroph PitNETs yet retain activity of NR5A1 (SF-1) and expression of some of SF-1-regulated genes (LHB and GNHRH). SF-1 apparently regulates slightly different set of genes in double positive somatotroph PitNETs and gonadotroph tumors. Based on scRNAseq data a subcluster of normal gonadotroph cells with POU1F1 (PIT-1) expression was identified, but tumor cells of PIT-1/SF-1 PitNETs were not similar to this subtype of normal gonadotrophs. A difference in genes expression profiles between 3 subtypes of somatotroph tumors was determined by analysis of both bulk- and scRNAseq data. From transcriptomic perspective of genes-coregulation and the activity of pituitary transcription factors, acromegaly-related PitNETs co-expressing PIT-1 and SF-1 are subtype of PIT-1 lineage tumors and molecular data do not support considering them as multilineage.