Archinuk, F.; Greenyer, H.; Stege, U.; Bennett, S. A. L.; Cuperlovic-Culf, M.; Jabbari, H.

Motivation: Various methods have been proposed to construct metabolic networks from metabolomic data; however, small sample sizes, multiple confounding factors, the presence of indirect interactions as well as randomness in metabolic processes are of major concern. Results: In this study, we benchmark existing algorithms for creating correlation- and regression-based networks of changes in metabolite abundance and we evaluate their performance across different sample sizes of a generative model. Using standard interaction-level tests and network-scale analyses based on centrality scores, we assess how well these methods capture simulated metabolomic networks. Our findings reveal significant challenges in network inference and result interpretation, even when sample sizes are significant and data are the result of computer modeling of metabolic pathways. Despite these limitations, we demonstrate that correlation-based network inference can, to some extent, discriminate between two different metabolic states. This suggests potential utility in distinguishing overarching changes in metabolic processes but not direct pathways in different conditions.