Silva, B. M.; Trindade, F. d. J.; Canesin, L. E. C.; Souza, G. B. S.; Aleixo, A.; Nunes, G. L.; Oliveira, R. R. M.

Motivation: With increasing initiatives to study biodiversity through high-quality reference genomes and the growing capacity of sequencing a wide range of organisms, there is a pressing need for an accessible, reproducible, and user-friendly tool that incorporates state-of-the-art methodologies for large genome assembly. Results: We introduce Pipeasm, a Snakemake pipeline designed for assembling vertebrate genomes using HiFi PacBio, ONT, and HiC data. By setting a configuration file with input information and suggested parameters, Pipeasm was able to assemble multiple-sized diploid genomes ready for manual curation. Availability and Implementation: Pipeasm requires an environment with Snakemake and Singularity and is available at https://github.com/itvgenomics/pipeasm.