Lorente Cobo, N.; Goyal, S.; Davidson, D.; Prehna, G.

The type VI secretion system (T6SS) is a dynamic nanomachine used by bacteria to compete for space and nutrients. To kill rival bacteria, the T6SS secretes toxic effector proteins directly into adjacent cells in a contact-dependent manner. Effectors have diverse biochemical functions that arise from subtle structural modifications to related enzyme folds. To protect from self-intoxication, effectors are encoded with a cognate immunity protein in effector-immunity pairs. Immunity proteins inhibit toxicity by directly binding the effector active site and are as structurally diverse as their effectors. Salmonella Typhimurium has several known effectors of diverse biochemical functions, however the effector-immunity pair Rhs2-SciX remains largely unstudied. Here, we take a structural approach and show that Rhs2 is a BECR family nuclease through modeling and toxicity assays. Furthermore, we solve an X-ray crystal structure of SciX and study the solution-state properties of the immunity protein to gain insight into the binding and inhibition mechanism of Rhs2. Finally, we discover that Rhs2 binds directly to EF-Tu suggesting that Rhs2 inhibits translation to cause cell death. Our work probes the structure and function of the effector-immunity pair Rhs2-SciX, demonstrates the biochemical diversity of Salmonella T6SS effectors, and highlights the conserved T6SS toxicity strategy of binding EF-Tu.