Singh, R.; Arfin, H. U.; Sharma, A.; KATERIYA, S.

Light is an essential key player in biomass and metabolite production in microalgae and the green lineage system. Light-sensing proteins (photoreceptors) can influence photosynthetic fitness by coordinating with the photosynthetic apparatus, thereby regulating biomass and various metabolic activities. However, genetic engineering is mainly employed for enhancing biomolecule production. The mechanism by which the different molecules and photoreceptors influence targeted metabolite accumulation under different light regimes is not clear. In the present study, in-silico and systems biology-based analysis, HPLC and label-free comparative quantitative proteomics were employed to investigate the imperative effect of different illumination conditions on metabolite production. The results indicated that blue light substantially enhances the accumulation of astaxanthin and chlorophyll via the blue light-sensor (photoreceptor). Thus, it suggests that fine-tuned illumination conditions influence molecular components in specific metabolite production. Moreover, crosstalk between photoreceptors with the protein family of carotenoid metabolic pathway, cell signalling, and identified biosynthetic gene clusters (BGCs) in green algae opens a new avenue in opto-biotechnology approaches for bioproducts production from other microalgae and the green lineage system.