Hanlon, M. B.; Wolfe, S. A.

Oncogene amplification is a key driver of tumorigenesis and a perpetuator of genomic instability. Oncogene amplification accelerates cancer cell proliferation and evolution, contributing substantially to the enhancement of adaptation mechanisms, such as treatment resistance, which pose a significant therapeutic challenge. However, previous studies have shown oncogene amplification to be a critical vulnerability, rendering cancer cells, but not normal cells, susceptible to targeted, CRISPR-Cas9 nickase - mediated DNA damage and cell death in vitro. Here, we demonstrate the initial framework for the translation of this potential therapeutic approach utilizing Cas9D10A - mRNA and functionalized lipid nanoparticles for the targeted delivery, and suppression of disseminated MYCN-amplified neuroblastoma in vivo.