Liu, Z.; Cordero, A.; Kinney, J. B.

Computationally designed DNA sequence libraries are essential components of many high-throughput assays. They are also increasingly used in silico to analyze genomic AI models. Designing these libraries, however, remains tedious and error-prone. Here we describe PoolParty, a Python package that streamlines the design of complex oligo pools using a simple but flexible API. In PoolParty, each library is represented by a computational graph that can be specified in just a few lines of code. Over 50 built-in operations cover nucleotide- and codon-level mutagenesis, motif insertion, barcode generation, and more. PoolParty also provides "design cards" detailing how each sequence was generated.