Ankley, P. J.; da Silva, F. C.; Roberts, C.; Eriksson, A. N. M.; Kohlman, E.; Dubiel, J.; Hunnie, B.; Anderson-Bain, K.; Urrutia, R. M.; Hogan, N.; Giesy, J. P.; Krol, E.; Wiseman, S.; Hecker, M.; Brinkmann, M.

N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6PPD-Q) is a ubiquitous and acutely toxic transformation product (TP) derived from the rubber tire antioxidant N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD). While not all salmonids are sensitive to acute lethality caused by 6PPD-Q, its potency can vary by several orders of magnitude among fish species. The main driver(s) of species sensitivity differences is a pressing question, with one area of interest examining whether differences in their ability to biotransform and detoxify 6PPD-Q could be a driving factor. This study utilized liquid-chromatography high-resolution mass spectrometry (LC-HRMS) to assess biotransformation and metabolome-wide effects of 6PPD-Q on early-life stage salmonids, including two sensitive species, rainbow trout (Oncorhynchus mykiss) and lake trout (Salvelinus namaycush), and one tolerant species, brown trout (Salmo trutta). Three phase I TPs and seven phase II TPs were detected, with differences in peak areas revealing that brown trout had the greatest ability to detoxify 6PPD-Q. TP-OH1, an alkyl sidechain monohydroxylated TP, was detected in both rainbow and lake trout but not in brown trout, with more research needed to understand potential TP-OH1 mediated toxicity. Several endogenous metabolites were found to be dysregulated in rainbow and lake trout, indicative of mitochondrial dysfunction and altered metabolism. Results of this study indicate a difference in the biotransformation capability of 6PPD-Q among salmonid fish species and subsequent unique metabolome responses.