Roske, Y.; Leidert, M.; Rehbein, K.; Diehl, A.

Filament-forming proteins such as TasA (Bacillus subtilis) and camelysins CalY1, CalY2 (Bacillus cereus) pose a particular challenge for structural analysis due to their strong tendency to self-association and their polydispersity, which severely limits their ability to crystallize or to be a target for NMR-spectroscopy. To address this, it is necessary to modify the amino acid sequence to prevent filamentation. Engineering a series of N- and C-terminal truncated variants by removing flexible parts is often key to success. N-terminal extensions are also a powerful tool for obtaining crystals of fiber-forming proteins.