Han, L.; Ji, Y.; Yu, Y.; Ni, Y.; Zeng, H.; Zhang, X.; Liu, H.; Yufeng, Z.

Osteoblast differentiation is crucial for bone formation and maintaining skeletal integrity. Although it is now understood that this process exhibits significant heterogeneity across developmental stages and tissue microenvironments, the underlying mechanisms remain largely unexplored. In the present study, we introduce TrajAtlas, a comprehensive framework that addresses this gap in knowledge. TrajAtlas comprises four modules: a reference atlas (Differentiation Atlas), a differentiation model (Differentiation model), a tool for differential pseudotime analysis (TrajDiff), and a method for pseudotemporal gene module detection (TRAVMap). By leveraging single-cell technologies, TrajAtlas offers a systematic approach to exploring the multi-scale heterogeneity among cells, genes, and gene modules within population-level trajectories across diverse tissues and age groups. We systematically investigate the impact of age and injury on osteogenesis, providing new insights into osteoporosis and bone regeneration. In conclusion, our comprehensive framework offers novel insights into osteogenesis and provides a valuable resource for understanding the complexities of bone formation.