Brown, G. G. B.; Gittens, W. H.; Allison, R. M.; Oliver, A. W.; Neale, M. J.

During meiosis Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5\' ends on both sides of the break during this process. Such Spo11 \"covalent complexes\" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronised Saccharomyces cerevisiae meiotic cells.