Young, J. C.; Lambert, A. R.; Young, J. M.; Doyle, L. A.; Silverstein, M.; Edgell, D. R.; Stoddard, B. L.

Homing endonucleases are selfish genetic elements that drive the mobilization of their own coding sequences, often in concert with surrounding introns. Homing endonuclease genes usually display life cycles in which they accumulate inactivating mutations after invading a host genomic target site, leading to eventual removal from the genome. We identified several hundred novel HEGs and determined the distribution of their behaviors and activities. Approximately ten percent are expressed as properly folded functional proteins that cleave predictable DNA target sites. Another approximately twenty percent display significant expression but little to no cleavage activity; the remainder display severely reduced expression. Despite the presence of debilitating mutations throughout most HEGs, ancestral reconstructions yielded endonucleases with improved expression and stability. One such reconstruction, at a hypothetical node preceding highly diverged HEs that cleave unique target sites, binds but does not cleave their individual targets. It instead cleaves a DNA sequence that represents a hybrid of those modern-day DNA targets while displaying a specificity profile that resembled those of previously characterized HEs. Its DNA-bound crystal structure adds detail to our understanding of how homing endonuclease DNA contacting surfaces and residues shift and rearrange during evolution, ultimately leading to their action at new target sites.