Making deep mutational scanning accessible: a cost-efficient approach to construct barcoded libraries for genes of any length
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Making deep mutational scanning accessible: a cost-efficient approach to construct barcoded libraries for genes of any length
Jann, J.; Gagnon-Arsenault, I.; Pageau, A.; Dube, A. K.; Fijarczyk, A.; Durand, R.; Landry, C. R.
AbstractRecent developments in DNA synthesis and sequencing have allowed the construction of comprehensive gene variant libraries and their functional analysis. Achieving high-replication and thorough mutation characterization remains technically and financially challenging for long genes. Here, we developed an efficient, affordable and scalable library construction approach that relies on low-cost DNA synthesis and standard cloning technologies, which will increase accessibility to systematic mutational studies and help advance the field of protein science.