Taniguti, L. M.; Vilanova, P. F.; Kitajima, J. P.; Monteiro-Vitorello, C. B.

In 2015, we published the complete genome assembly of Sporisorium scitamineum, the fungal pathogen responsible for sugarcane smut disease, generated primarily from PacBio long-read sequencing and polished with Illumina short reads. Since then, the tools for genome assembly have improved considerably, including advances in sequencing technologies and bioinformatics workflows, motivating us to revisit our original assembly. Here, we present an updated genome version, re-polished using newly generated high-quality Illumina short-read data and state-of-the-art variant-calling workflows, resulting in corrections at approximately 5,500 genomic positions, predominantly correcting SNP and indel errors. BUSCO benchmarking demonstrated increased genome completeness from the original 94.2% to 99.3%, indicating substantial improvements in both accuracy and gene annotation reliability. This enhanced genome assembly provides a significantly improved reference resource, enabling more robust genetic, evolutionary, and functional studies of this economically important sugarcane pathogen.