An mTurquoise2-based glucose biosensor
An mTurquoise2-based glucose biosensor
Botman, D.; Tielman, A.; Goedhart, J.; Teusink, B.
AbstractGlucose is an important substrate for organisms to acquire energy needed for cellular growth. Despite the importance of this metabolite, single-cell information at a fast time-scale about the dynamics of intracellular glucose levels is difficult to obtain as the current available sensors have drawbacks in terms of pH sensitivity or glucose affinity. To address this, we developed a convenient method to make and screen biosensor libraries using yeast as workhorse. This resulted in TINGL (Turquoise INdicator for GLucose), a robust and specific biosensor for intracellular glucose detection. We calibrated the sensor in vivo through equilibration of internal and external glucose in a yeast mutant unable to phosphorylate glucose. Using this method, we measured dynamic glucose levels in budding yeast during transitions to glucose. We found that glucose concentrations reached levels up to 1 mM as previously determined biochemically. Furthermore, the sensor showed that intracellular glucose dynamics differ based on whether cells are glucose-repressed or not. We believe that this sensor can aid researchers interested in cellular carbohydrate metabolism.