Zimmann, F.; Banik, P.; Kubovciak, J.; Thakur, P. K.; Capek, M.; Kolar, M.; Hruba, E.; Dobrovolny, R.; Cvackova, Z.; Barta, T.; Stanek, D.

Retinitis pigmentosa (RP) is an inherited retinal disorder characterized by the progressive loss of photoreceptors that currently lacks effective treatment. Here, we investigated the impact of the pathogenic PRPF8-Y2334N variant on neural retina cells in hiPSC-derived retinal organoids. Expression of this variant resulted in photoreceptor defects, including thinning of the outer segment layer. At the molecular level, we observed relatively minor changes in mRNA expression in multiple retinal cells, indicating that neural retina cells are impacted independently of retinal pigment epithelium (RPE). We found splicing alterations in genes associated with neural and retinal diseases, including those involved in intraflagellar transport, suggesting that these genes may represent common targets of splicing factor mutations. We further detected the misexpression of several circular RNAs (circRNAs), which could serve as early biomarkers of splicing defects caused by RP mutations. Together, we present a model of RP that recapitulates photoreceptor degeneration and demonstrates that these defects are independent of RPE erosion.