Alzyoud, E.; Nemeth, D.; Vedelek, V.; Szögi, T.; Krecsmarik, M.; Lipinszki, Z.; Sinka, R.

The initiation of microtubule formation in eukaryotes is primarily facilitated by {gamma}-tubulin and the highly conserved protein complex called {gamma}-Tubulin Ring Complex ({gamma}-TuRC) in various microtubule-organizing centers (MTOCs). Non-centrosomal MTOCs have a range of functions and contribute to diverse cellular activities in different cell types. While the heterogeneity of tissue-specific MTOCs and {gamma}-TuRC in Drosophila testis is known, their molecular composition and physiological significance are poorly understood. In this study, we investigate the testis-specific distribution of the canonical {gamma}-TuRC by in vivo tagging of two complex members, Grip163 and Grip84. We find that both the testis-specific (t- {gamma}-TuRC) and canonical form of {gamma}-TuRC are simultaneously present and colocalize with each other at the basal body, apical nuclear tip, and near the mitochondrial derivatives in the post-meiotic spermatids. We propose the possibility of the formation of MTOCs containing both t-{gamma}-TuRC and {gamma}-TuRC complexes. We find some of the known {gamma}-TuRC binding partners localizing to the apical nuclear tip. Additionally, we identify the Golgi localization of Grip84 in spermatocytes, alongside its centrosomal and diverse post-meiotic localization. These results highlight and prove the importance of the different {gamma}-TuRCs in organizing the diverse MTOCs during the extensive rearrangement of cell organelles in Drosophila spermatogenesis.