GLON, D.; Riller, Q.; Riviere, F.; Leonardon, B.; Guillemot, A.; Sago, L.; Pelle, O.; Ho-Nhat, D.; Brochard, K.; Bader-Meunier, B.; Fremond, M.-L.; Lepelley, A.; Crow, Y.; Tusseau, M.; Belot, A.; Lagaudriere-Gesbert, C.; Rieux-Laucat, F.; GAUDIN, Y.

TBK1 kinase is a central regulator of type I IFN production. Upon activation of the IFN-{beta} induction pathway, TBK1-adaptor proteins (NAP1, SINTBAD, TANK) form condensates with liquid properties. We showed that NAP1 condensates concentrate TBK1. Using NAP1KO cell lines, we discovered that NAP1 exerts a dual effect on TBK1 activity. Initially, NAP1 binds TBK1 and increases its activity, which enhances the activation of the IFN pathway. Then, phosphorylation of NAP1 by TBK1 induces the formation of NAP1 condensates. These condensates concentrate TBK1 and PP2A, a phosphatase known to dephosphorylate and consequently deactivate TBK1, thus limiting IFN induction. Additionally, in patients with lupus or interferonopathies, we identified NAP1 variants, unable to form condensates upon cell exposure to danger signals, which can only activate TBK1 without limiting its activity. This study reveals an original mode of regulating a signaling pathway by formation of condensates and provides a molecular explanation for certain interferonopathies.