Assad, Z.; La, k.; Levy, C.; Cohen, R.; El Mniai, A.; Fafi, I.; Valtuille, Z.; Bechet, S.; Corrard, F.; Werner, A.; Rodriguez, C.; N'Debi, M.; Lo, S. W.; Blanquart, F.; Osei, L.; Jaboyedoff, M.; Angoulvant, F.; Bonacorsi, S.; Bidet, P.; Birgy, A.; de Pontual, L.; Basmaci, R.; Varon, E.; Morel-Journel, T.; Ouldali, N.

Streptococcus pneumoniae is a colonizer of the child s nasopharynx and a leading cause of invasive pneumococcal disease (IPD). Despite widespread use of pneumococcal conjugate vaccines, recent rebounds in IPD incidence have coincided with serotype replacement. Whether this phenomenon is driven by the replacement of serotypes within lineages or the replacement of the lineages themselves is unclear. Here, we quantified the relative contribution of these two mechanisms to serotype replacement in carriage and their consequences for IPD. We combined nationwide longitudinal surveillance of carriage and IPD in children from 2002 to 2023 with whole-genome sequencing. Observed lineage-specific carriage rates across PCV-related periods were fitted with polynomial logistic regression and compared with two counterfactual scenarios, each isolating one mechanism with: (i) fixed serotype composition within varying lineages and (ii) serotype shifts within fixed lineage carriage rates over time. Carriage dynamics were consistent with an 82% contribution of serotype shifts within persistent lineages. Estimates of serotype-specific IPD incidence derived from this predominant scenario correlated with observed IPD trends. These findings quantify the dominant role of serotype replacement within persistent lineages, disentangling the vaccine-driven adaptive history of S. pneumoniae.