Heath, G. R.; Micklethwaite, E.; Storer, T. M.

NanoLocz is an open-source computer program designed for high-throughput automatic processing and single-particle analysis of Atomic Force Microscopy (AFM) image data. High-Speed AFM and Localization AFM (LAFM) enable the study of single molecules with increasingly higher spatiotemporal resolution. However, efficient and rapid analysis of the images and movies produced by these techniques can be challenging, often requiring the use of multiple image processing software applications and scripts. Here, we introduce NanoLocz, an AFM and high-speed AFM analysis program that facilitates various single-particle analysis workflows through a simple, interactive interface. Workflows include but are not limited to: single-particle tracking, single-particle topographic feature analysis, single-molecule LAFM, time-resolved LAFM, and simulation LAFM. The source code and installation instructions for NanoLocz are available at https://github.com/George-R-Heath/NanoLocz.