Rearing, dissection, and temporal transcriptomic profiling protocols to study density-dependent phenotypic plasticity in Schistocerca (Insecta: Orthoptera)
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Rearing, dissection, and temporal transcriptomic profiling protocols to study density-dependent phenotypic plasticity in Schistocerca (Insecta: Orthoptera)
Techer, M. A.; Santana, V. A. P.; Woo, B.; Marquess, R.; Brennan, C.; Mechti, A. M. C.; Linde, J. B.; Behmer, S. T.; Sword, G. A.; Song, H.
AbstractThis protocol generates gregarious and solitarious density-dependent phenotypes in multiple Schistocerca species under controlled environmental conditions. It describes cage setup, feeding, animal handling, and sterile dissection workflows to isolate nervous, chemosensory, gut, fat body, and female reproductive tissues from nymphs and adults. It emphasizes rapid tissue stabilization and RNase-control practices for downstream single-tissue DNA and RNA analyses.