Highly-sensitive reporter cell line for detection of interferon types I-III and their neutralization by antibodies
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Highly-sensitive reporter cell line for detection of interferon types I-III and their neutralization by antibodies
Groen, K.; Kuratli, R.; Vasou, A.; Huber, M.; Hughes, D. J.; Hale, B. G.
AbstractInterferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly-used alternative biological reporter system, and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.