Castilla Bolanos, M. A.; Chinchalongporn, V.; Ghosh Biswas, R.; Bailey, C.; Wu, M.; Soong, R.; Saleh, F.; Simpson, A.; Schuurmans, C.; Near, J.

Human-derived cerebral organoids (COs) are an emerging model system for the study of human neural development and physiology. Here, we describe the assessment of metabolism in human-derived COs using high-resolution magic-angle spinning (HR-MAS) NMR spectroscopy. Metabolic changes during development are assessed by studying COs at various stages of maturity. Our results suggest that COs exhibit a metabolic profile similar to in vivo human brain metabolism, albeit with a few notable metabolic differences.