Passalacqua, M. J.; Gillis, J.

Single-cell RNA sequencing is increasingly used to investigate cross-species differences driven by gene expression and cell-type composition in plants. However, the frequent expansion of plant gene families due to whole genome duplications makes identification of one-to-one orthologs difficult, complicating integration. Here, we demonstrate that coexpression can be used to identify non-orthologous gene pairs with proxy expression profiles, improving the performance of traditional integration methods and reducing barriers to integration across a diverse array of plant species.