Kraftberger, M.; Spirgath, K.; Haase, T.; Bandelin, R.; Meyer, T.; Jaitner, N.; Tzschätzsch, H.

Arterial vascular diseases, such as atherosclerosis, are among the most serious global health threats. In preclinical studies, morphometric analysis of histological arterial cross-sections is considered the gold standard for assessing vascular remodeling and the effectiveness of therapeutic interventions. However, morphometric analysis is usually performed manually, which is time-consuming, subjective, and requires significant user interaction. This paper presents a fully automated, operator-independent framework for the precise morphometric analysis of stented arterial cross-sections, extending the previously developed qHisto (quantitative histology) framework for the quantification of various histological components. A neural network for the segmentation of arterial structures was trained and evaluated using 819 cross-sections. In addition, a quantitative analysis of vascular morphology, fibrin area, and lumen asymmetry was performed using 72 cross-sections from coated and uncoated balloons. The model achieved high segmentation accuracy with a median Dice similarity coefficient of 0.892-0.996. Compared to manual evaluation, the system reduces analysis time by 90%, enabling efficient processing of large datasets. Furthermore, morphometric analysis with qHisto showed significant differences between coated and uncoated balloons, e.g. regarding lumen area (AUC = 0.86) and fibrin ratio (AUC = 0.94). Our developed framework enables fully automated, comprehensive and standardized analysis of histological arterial cross-sections. This helps to reduce time-consuming, repetitive manual assessments and thus facilitates research of disease mechanisms and treatment effects in preclinical studies.