Le Compte, G.; Ceylan, H.; Meysman, P.; Laukens, K.

Chimeric Antigen Receptors (CARs) are modular synthetic constructs that have transformed cellular immunotherapy, enabling targeted recognition and killing of malignant cells. Their clinical success has driven an explosive growth in new receptor designs, but these sequences are dispersed across heterogeneous sources such as publications, patents and supplementary files. This fragmentation and inconsistency limits comparative analysis, reproducibility and the reuse of existing constructs. To address this, we curated and standardized more than 10,000 CAR sequences into a single, harmonized resource. CARTiBASE is a web-based platform that provides standardized annotation, interactive browsing and fast similarity search across this curated collection. Users can search and filter thousands of CARs, inspect domain boundaries across signal peptide, antigen-binding domain, hinge, transmembrane, co-stimulatory and intracellular signalling regions, compare constructs and download sequences as FASTA files for downstream use.